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Figures and Supplemental Data for GloFish Experiments

Supplemental Figures (raw data)

Supplemental Figure Legends

Supplemental Figure 1: Progeny of a dihybrid cross between a non-striped, yellow female GloFish and a wildtype male zebrafish

Supplemental Figure 2: Progeny of a dihybrid cross between two parents heterozygous for GloYFP and gol

Supplemental Figure 3: Progeny of a trihybrid cross between a yellow GloFish and red GloFish, both heterozygous for gol

Supplemental Figure 4: Progeny of cross between fish heterozygous for the Glo transgene and lof mutation

Supplemental Figure 5: Progeny of cross between two cyc heterozygotes

Supplemental Figure 6: Progeny of cross between two sqt heterozygotes

Supplemental Figure 7: Cross between two WT fish to determine inheritance of gender

Supplemental Figure 8: Hypothesis for gender inheritance pattern

Supplemental Figure 9: Progeny of cross between two wildtype fish

Supplemental Tables

Supplemental Materials (for instructors)

Supplemental Material 1: Animal Training Homework

Supplemental Material 2: Instructors' Key for GloFish Laboratory

Supplemental Material 3: PPT Introduction to GloFish Laboratory

Supplemental Material 4: Laboratory Handout for GloFish Laboratory

Supplemental Material 5: Laboratory Worksheet for GloFish Laboratory

Supplemental Material 6: Homework for GloFish Laboratory

Supplemental Material 7: Instructions and Rubric for Laboratory Notebook

Supplemental Material 8: Instructors' Key for F3 Genetic Screen Laboratory

Supplemental Material 9: PPT Introduction to F3 Genetic Screen Laboratory

Supplemental Material 10: Laboratory Handout for F3 Screen Laboratory

Supplemental Material 11: Chi-square Worksheet

Supplemental Material 12: F3 Screen Homework (easier)

Supplemental Material 13: F3 Screen Homework (harder)